BID Antibody

Purified Mouse Monoclonal Antibody (Mab)

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All lanes : Anti-BID Antibody at 1:500-2000 dilution Lane 1: HeLa whole cell lysate Lane 2: A431 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: Raji whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling BID with AM8474b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
AM8474b staining BID in human bladder sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-BID Antibody at 1:4000 dilution Lane 1: Jurkat whole cell lysates Lane 2: A431 whole cell lysates Lane 3: THP-1 whole cell lysates Lane 4: 293T/17 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, E
Primary Accession	P55957
Reactivity	Human
Host	Mouse
Clonality	monoclonal
Isotype	IgG1,k
Clone Names	1519CT649.18.47
Calculated MW	21995 Da

Additional Information
Gene ID	637
Other Names	BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, BID
Target/Specificity	This BID antibody is generated from a mouse immunized with a recombinant protein of human BID.
Dilution	WB~~1:4000 IHC-P~~1:100~500 IF~~1:25 E~~Use at an assay dependent concentration.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	BID Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	BID
Function	Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity).
Cellular Location	Cytoplasm. Mitochondrion membrane. Mitochondrion outer membrane. Note=When uncleaved, it is predominantly cytoplasmic. [BH3-interacting domain death agonist p13]: Mitochondrion membrane {ECO:0000250\|UniProtKB:P70444}. Note=Associated with the mitochondrial membrane. {ECO:0000250\|UniProtKB:P70444} [Isoform 3]: Cytoplasm
Tissue Location	[Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level). [Isoform 4]: Expressed in lung and pancreas (at protein level).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

REFERENCES

Wang K.,et al.Genes Dev. 10:2859-2869(1996).
Footz T.K.,et al.Genomics 51:472-475(1998).
Renshaw S.A.,et al.J. Biol. Chem. 279:2846-2855(2004).
Dai F.Y.,et al.Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).

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¥ 1,250.00

Cat# AM8474b

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BID Antibody

Purified Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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