> 首页 > 产品 > 一抗 > 细胞生物学 > FAM120A Antibody [Knockdown Validated]

FAM120A Antibody [Knockdown Validated]

Purified Mouse Monoclonal Antibody (Mab)

All lanes: Anti-FAM120A Antibody at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: U-2OS whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated (ASP1613) at 1/8000 dilution. Observed band size: 135 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling FAM120A with AM8475b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
AM8475b staining FAM120A in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-FAM120A Antibody at 1:2000 dilution Lane 1: Hela Lane 2: Hela-Knockdown Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated (ASP1613) at 1/8000 dilution.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, E
Primary Accession	Q9NZB2
Reactivity	Human
Host	Mouse
Clonality	monoclonal
Isotype	IgG1,k
Clone Names	1501CT803.19.85.5
Calculated MW	121888 Da

Additional Information
Gene ID	23196
Other Names	Constitutive coactivator of PPAR-gamma-like protein 1, Oxidative stress-associated Src activator, Protein FAM120A, FAM120A, C9orf10, KIAA0183, OSSA
Target/Specificity	This FAM120A antibody is generated from a mouse immunized with a recombinant protein of human FAM120A.
Dilution	WB~~1:2000 IHC-P~~1:100~500 IF~~1:25 E~~Use at an assay dependent concentration.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	FAM120A Antibody [Knockdown Validated] is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	FAM120A
Function	Component of the oxidative stress-induced survival signaling. May regulate the activation of SRC family protein kinases (PubMed:19015244). May act as a scaffolding protein enabling SRC family protein kinases to phosphorylate and activate PI3-kinase (PubMed:19015244). Binds IGF2 RNA and promotes the production of IGF2 protein (PubMed:19015244).
Cellular Location	Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Translocates from the cytosol to plasma membrane after UV irradiation.
Tissue Location	Widely expressed (PubMed:14585507). In gastric mucosa, detected in the bottom region of the foveolar epithelium (at protein level) (PubMed:19015244).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous- type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.

REFERENCES

Brahmbhatt S.B.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).
Nagase T.,et al.DNA Res. 3:17-24(1996).
Ohara O.,et al.Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
Holden S.,et al.Gene 318:149-161(2003).

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.

我要评价

¥ 1,250.00

Cat# AM8475b

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

Quantity:

reduce add

Availability: In Stock

询价

FAM120A Antibody [Knockdown Validated]

Purified Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

相关产品

多肽合成服务

多克隆抗体制备服务

单克隆抗体制备服务

抗体测序服务

蛋白表达服务

药物发现定制服务