注册 | 登录
点击这里给我发消息
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   细胞生物学   >   FAM120A Antibody   

FAM120A Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - FAM120A Antibody AM8475b
    All lanes: Anti-FAM120A Antibody at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: U-2OS whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated (ASP1613) at 1/8000 dilution. Observed band size: 135 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - FAM120A Antibody AM8475b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling FAM120A with AM8475b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
  • 14 - FAM120A Antibody AM8475b
    AM8475b staining FAM120A in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 产品详情
  • 实验流程
  • 背景知识
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession Q9NZB2
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1501CT803.19.85.5
Calculated MW 121888 Da
Additional Information
Gene ID 23196
Other Names Constitutive coactivator of PPAR-gamma-like protein 1, Oxidative stress-associated Src activator, Protein FAM120A, FAM120A, C9orf10, KIAA0183, OSSA
Target/Specificity This FAM120A antibody is generated from a mouse immunized with a recombinant protein of human FAM120A.
Dilution WB~~1:1000
IHC-P~~1:100~500
IF~~1:25
E~~Use at an assay dependent concentration.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFAM120A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FAM120A
Function Component of the oxidative stress-induced survival signaling. May regulate the activation of SRC family protein kinases (PubMed:19015244). May act as a scaffolding protein enabling SRC family protein kinases to phosphorylate and activate PI3-kinase (PubMed:19015244). Binds IGF2 RNA and promotes the production of IGF2 protein (PubMed:19015244).
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Translocates from the cytosol to plasma membrane after UV irradiation.
Tissue Location Widely expressed (PubMed:14585507). In gastric mucosa, detected in the bottom region of the foveolar epithelium (at protein level) (PubMed:19015244).
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous- type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.

REFERENCES

Brahmbhatt S.B.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).
Nagase T.,et al.DNA Res. 3:17-24(1996).
Ohara O.,et al.Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
Holden S.,et al.Gene 318:149-161(2003).

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价