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ETS1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 4 - ETS1 Antibody AM8521b
    Overlay histogram showing K562 cells stained with AM8521b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8521b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - ETS1 Antibody AM8521b
    Anti-ETS1 Antibody at 1:500 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P14921
Reactivity Human, Rat, Mouse
Host Mouse
Clonality monoclonal
Isotype IgG2b,k
Clone Names 1601CT512.35.61
Calculated MW 50408 Da
Additional Information
Gene ID 2113
Other Names Protein C-ets-1, p54, ETS1, EWSR2
Target/Specificity This ETS1 antibody is generated from a mouse immunized with a recombinant protein of human ETS1.
Dilution WB~~1:500
FC~~1:25
E~~Use at an assay dependent concentration.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsETS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ETS1
Synonyms EWSR2
Function Transcription factor (PubMed:10698492, PubMed:11909962). Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed:20378371). May control the differentiation, survival and proliferation of lymphoid cells (PubMed:20378371). May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed:15247905, PubMed:15592518).
Cellular Location Nucleus. Cytoplasm Note=Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.
Tissue Location Highly expressed within lymphoid cells. Isoforms c- ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.

REFERENCES

Reddy E.S.P.,et al.Oncogene Res. 3:239-246(1988).
Watson D.K.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:7862-7866(1988).
Laitem C.,et al.Oncogene 28:2087-2099(2009).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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