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PFKP Antibody (C-term) 精选

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - PFKP Antibody (C-term) AM8580b
    All lanes : Anti-PFKP Antibody (C-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: COS-7 whole cell lysate Lane 4: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q01813
Reactivity Human, Green Monkey, Mouse
Predicted Rat, Bovine, Canine, Rabbit, Chicken
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1040CT4.6.4.4
Calculated MW 85596 Da
Additional Information
Gene ID 5214
Other Names ATP-dependent 6-phosphofructokinase, platelet type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-P, 2.7.1.11 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, PFK-C, Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}, PFKP, PFKF
Target/Specificity This PFKP antibody is generated from a mouse immunized with a recombinant protein between 40-300 amino acids from the C-terminal region of human PFKP.
Dilution WB~~1:4000
E~~Use at an assay dependent concentration.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPFKP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name PFKP (HGNC:8878)
Synonyms PFKF
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}.
Research Areas

BACKGROUND

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

REFERENCES

Eto K.,et al.Biochem. Biophys. Res. Commun. 198:990-998(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).
Simpson C.J.,et al.Biochem. Biophys. Res. Commun. 180:197-203(1991).
Rush J.,et al.Nat. Biotechnol. 23:94-101(2005).

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