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RAB5B Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - RAB5B Antibody AM8689b
    Anti-RAB5B Antibody at 1:2000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - RAB5B Antibody AM8689b
    All lanes : Anti-RAB5B Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: NIH-3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P61020
Other Accession P61021, Q5RBG1
Reactivity Human, Mouse
Predicted Mouse
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1615CT175.80.21
Calculated MW 23707 Da
Additional Information
Gene ID 5869
Other Names Ras-related protein Rab-5B, RAB5B
Target/Specificity This RAB5B antibody is generated from a mouse immunized with a recombinant protein of human RAB5B.
Dilution WB~~1:2000
E~~Use at an assay dependent concentration.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB5B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB5B (HGNC:9784)
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Note=Enriched in stage I melanosomes
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Protein transport. Probably involved in vesicular traffic (By similarity).

REFERENCES

Wilson D.B.,et al.J. Clin. Invest. 89:996-1005(1992).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Scherer S.E.,et al.Nature 440:346-351(2006).

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