癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Phospho-Ser482,486,490 Polo-Like Kinase Kinase Antibody
Affinity purified rabbit polyclonal antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB |
|---|---|
| Primary Accession | P70032 |
| Reactivity | Xenopus |
| Predicted | Human, Mouse, Rat, Zebrafish |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 68212 Da |
| Gene ID | 380481 |
|---|---|
| Gene Name | PLK1 |
| Other Names | Serine/threonine-protein kinase PLK1, Plx1, Polo-like kinase 1, PLK-1, plk1, plx1 |
| Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 conjugated to KLH. |
| Dilution | WB~~1:1000 |
| Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
| Antibody Specificity | Specific for the ~120k PLKK protein phosphorylated at Ser482,486,490. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-Ser482,486,490 Polo-Like Kinase Kinase Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.
REFERENCES
Erikson E, Haystead TAJ, Qian, Y-W, Maller JL (2004) A feedback loop in the polo-like kinase activation pathway. J Biol Chem 279:32219-32224.
Kumagai A, Dunphy WG (1996) Purification and molecular cloning of Plx1, a cdc25-regulatory kinase from Xenopus egg extracts. Science 273:1377-1380.
Liu J, Lewellyn AL, Chen LG, Maller JL (2004) The polo box is required for multiple functions of Plx1 in mitosis. J Biol Chem 279:21367-21373.
van Vugt, MATM, van de Weerdt, BCM, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RMF, Medema RH (2004) Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/cdc20 activation and initiation of cytokinesis. J Biol Chem 279:36841-36854.
Eleanor Erikson, Timothy A. J. Haystead, Yue-Wei Qian, and James L. Maller (2004) A Feedback Loop in the Polo-like Kinase Activation Pathway. J. Biol. Chem., Jul 2004; 279: 32219 - 32224
终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问，联系：0512-88856768 tech-china@abcepta.com.
