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Anti-Actin Antibody

Our Anti-Actin primary antibody from PhosphoSolutions is mouse monoclonal. It detects all known spec

     
  • 0 - Anti-Actin Antibody AN1306
    Western blot of rat hippocampal lysate showing specific immunolabeling of the ~42 kDa actin protein.
  • 0 - Anti-Actin Antibody AN1306
    Immunofluorescence staining of human blood-brain barrier endothelial cells, HMEC 3, showing specific labeling of actin (cat. AN1306, red, 1:200) The cell nuclei are stained in blue with DAPI. Photo credit of Yancy Ferrer-Acosta/Henrique Martins, Universidad Central Del Caribe, PR.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, ICC
Primary Accession P68139
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names C4
Calculated MW 42051 Da
Additional Information
Gene ID 421534
Other Names a actin antibody, ACTA antibody, ACTA1 antibody, Actin alpha skeletal muscle antibody, Actin antibody, actin alpha 1 skeletal muscle 1 antibody, actin alpha 1 skeletal muscle antibody, Actin alpha skeletal muscle antibody, actina antibody, actine antibody, ACTS_HUMAN antibody, aktin antibody, Alpha Actin 1 antibody, Alpha skeletal muscle Actin antibody, alpha skeletal muscle antibody, alpha-actin antibody, Alpha-actin-1 antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, nemaline myopathy type 3 antibody
Target/Specificity Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.
Dilution WB~~1:1000
IHC~~1:100~500
ICC~~N/A
Format Mouse ascites fluid
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAnti-Actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.

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