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Anti-Phosphoserine/threonine Antibody

     
  • 0 - Anti-Phosphoserine/threonine Antibody AN1899
    Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.
  • 0 - Anti-Phosphoserine/threonine Antibody AN1899
    Bar graph showing anti-Phosphoserine/threonine (AN1899) binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC, IP
Primary Accession N/A
Host Rabbit
Clonality Rabbit Polyclonal
Isotype IgG
Additional Information
Other Names Phosphoser/thr mAb
Dilution WB~~1:1000
ICC~~N/A
IP~~N/A
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAnti-Phosphoserine/threonine Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

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