RB Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Immunohistochemical staining of paraffin-embedded human normal esophagus (A) and stomach (B) tissue, showing nucleus localization using Rb mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC, E
Primary Accession	P06400
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7E4B8
Isotype	IgG1
Calculated MW	106159 Da
Description	The Rb protein regulates differentiation, apoptosis, and cell cycle control by coordinating the cell cycle at G1-S with transcriptional machinery. During G1, cyclin D-dependent kinase-mediated phosphorylation of Rb at Ser-795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues. Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene(Rb).
Immunogen	Purified recombinant fragment of human RB expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Other Names	Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1
Dilution	IHC~~1/200 - 1/1000 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.