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ELK1 Antibody

Purified Mouse Monoclonal Antibody

     
  • 1 - ELK1 Antibody AO1080a
    Figure 1: Western blot analysis using ELK1 mouse mAb against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).
  • 2 - ELK1 Antibody AO1080a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human smooth musde sarcoma, showing cytoplasmic localization using Desmin mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P19419
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7E10D5; 7E10E8
Isotype IgG1
Calculated MW 44888 Da
Description The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.
Immunogen Purified recombinant fragment of ELK1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2002
Other Names ETS domain-containing protein Elk-1, ELK1
Dilution WB~~1/500 - 1/2000
E~~N/A
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsELK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ELK1 (HGNC:3321)
Function Transcription factor that binds to purine-rich DNA sequences (PubMed:10799319, PubMed:7889942). Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2 (PubMed:1630903). Induces target gene transcription upon JNK and MAPK- signaling pathways stimulation (PubMed:7889942).
Cellular Location Nucleus.
Tissue Location Lung and testis.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

1. Rao,V.N., et al. 1989.Science.244 (4900):66-70. 2. Hsieh,Y.H., et al. 2006.Biochem. Biophys. Res. Commun. 339 (1): 217-225. 3. Gille,H., Strahl,T. and Shaw,P.E.1995. Curr. Biol. 5 (10): 1191-1200. 4. Gille,H., et al. 1995.EMBO J. 14 (5): 951-962.

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