癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Thioredoxin (TRX) Antibody
Purified Mouse Monoclonal Antibody
- 产品详情
- 实验流程
Application 
| WB, E |
|---|---|
| Primary Accession | P10599 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1H6H6 |
| Isotype | IgG1 |
| Calculated MW | 11737 Da |
| Description | Thioredoxin (TRX) is a small ubiquitous protein (MW12kDa) which is exist in a wide variety of prokaryotic and eukaryotic cells. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. This antibody is suitable for detecting fusion proteins which encode a Trx-Tag by immunoblotting and immunoprecipitation. The Monoclonal Antibody can detect a little Trx-Tag fusion proteins with negligible cross-reactivity with bacterial, insect, or mammalian lysates. |
| Immunogen | Purified recombinant fusion protein with Thioredoxin (TRX) tag. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 7295 |
|---|---|
| Other Names | Thioredoxin, Trx, ATL-derived factor, ADF, Surface-associated sulphydryl protein, SASP, Hom s Trx, TXN, TRDX, TRX, TRX1 |
| Dilution | WB~~1/500 - 1/2000 E~~N/A |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Thioredoxin (TRX) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TXN |
|---|---|
| Synonyms | TRDX, TRX, TRX1 |
| Function | Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed:17182577, PubMed:19032234, PubMed:2176490). Plays a role in the reversible S- nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed:16408020, PubMed:17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed:11118054, PubMed:9108029). |
| Cellular Location | Nucleus. Cytoplasm. Secreted Note=Translocates from the cytoplasm into the nucleus after phorbol 12- myristate 13-acetate induction (PMA) (PubMed:9108029). Predominantly in the cytoplasm in non irradiated cells (PubMed:11118054). Radiation induces translocation of TRX from the cytoplasm to the nucleus (PubMed:11118054). Secreted by a leaderless secretory pathway (PubMed:1332947). |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
REFERENCES
1. Holmgen, A. et al., Annu. Rev. Biochem. 54, 237-271 (1985). 2. Wollman, E. E. et al., J. Biol. Chem. 263, 15506-15512 (1988). 3. Sasada, T. et al., J. Toxicol. Sci. 21, 285-287 (1996).
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