GFP Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).
Figure 2: Western blot analysis using GFP mouse mAb against extracts from HCC827 cells, untransfected (1) and transfected with GFP(2).
Figure 3: Immunocytochemistry analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb .
Figure 4: Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb .

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Host	Mouse
Clonality	Monoclonal
Clone Names	4B10B2
Isotype	IgG2a
Calculated MW	27 KDa
Description	GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Immunogen	Purified recombinant fragment of GFP expressed in E. Coli.
Formulation	Purified antibody in PBS containing 0.03% sodium azide.

Additional Information
Dilution	WB~~1/500 - 1/2000 IHC~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GFP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

1. Proc Natl Acad Sci U S A. 2006, Jul 25,103(30):11399-404. 2. Gene Expr Patterns. 2007, Jan,7(1-2):124-30. 3. Eur J Cell Biol. 2006, Sep,85(9-10):1059-68.

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¥ 1,250.00

Cat# AO1117a

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

Quantity:

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Availability: 3-5 days

询价

GFP Antibody

Purified Mouse Monoclonal Antibody

REFERENCES

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