HDAC3 Antibody

Mouse Monoclonal Antiobody to HDAC3

Figure 1: Western blot analysis using HDAC3 mouse mAb against truncated Trx-HDAC3 recombinant protein (1), full length HDAC3-hIgGFc (aa1-428) transfected CHO-K1 cell lysate(2) and Hela cell lysate (3).
Figure 2: Immunohistochemical analysis of paraffin-embedded human esophagus cancer (left) and breast carcinoma tissue (right), showing nuclear localization with DAB staining using HDAC3 mouse mAb.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	O15379
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3A7B5
Isotype	Mouse IgG1
Calculated MW	48848 Da
Description	HDAC3: histone deacetylase 3, also known as HD3, RPD3, RPD3-2. Entrez Protein NC_000005. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
Immunogen	Purified recombinant fragment of HDAC3(aa224-428) expressed in E. Coli.

Additional Information
Other Names	Histone deacetylase 3, HD3, RPD3-2, SMAP45, HDAC3
Target/Specificity	Purified recombinant fragment of HDAC3(aa224-428) expressed in E. Coli.
Dilution	WB~~1:500~~2000 IHC~~1:200~~1000 E~~N/A
Format	Ascitic fluid containing 0.03% sodium azide.
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	HDAC3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.