STAT3 Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using STAT3 mouse mAb against full-length STAT3-His recombinant protein (1).
Figure 2: Immunohistochemical analysis of paraffin-embedded human Liver tissues using STAT3 mouse mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P40763
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7G3H4
Isotype	IgG1
Calculated MW	88068 Da
Description	STAT3: signal transducer and activator of transcription 3 (acute-phase response factor). The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
Immunogen	Purified recombinant fragment of STAT3 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Other Names	Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3, APRF
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	STAT3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.