癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Proteinase 3 Antibody
Purified Mouse Monoclonal Antibody
- 产品详情
- 实验流程
Application 
| WB, E |
|---|---|
| Primary Accession | P24158 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 3B4 |
| Isotype | IgG2b |
| Calculated MW | 27807 Da |
| Description | Proteinase 3, also known as PRTN3. PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown but in human neutrophils proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis. |
| Immunogen | Purified recombinant fragment of human Proteinase 3 expressed in E. Coli. |
| Formulation | Antibody are purified by protein G affinity chromatography. Liquid in PBS containing 50% glycerol and 0.03% sodium azide. |
| Gene ID | 5657 |
|---|---|
| Other Names | Myeloblastin, 3.4.21.76, AGP7, C-ANCA antigen, Leukocyte proteinase 3, PR-3, PR3, Neutrophil proteinase 4, NP-4, P29, Wegener autoantigen, PRTN3, MBN |
| Dilution | WB~~1/500 - 1/2000 E~~N/A |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Proteinase 3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PRTN3 |
|---|---|
| Synonyms | MBN |
| Function | Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed:2033050, PubMed:28240246, PubMed:3198760). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed:23202369). Plays a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed:22266279). Triggers inflammatory processes in neutrophils by interacting with ADGRG3 upstream of F2RL1/PAR2 activation (PubMed:36302784). |
| Cellular Location | Cytoplasmic granule. Secreted. Cell membrane; Peripheral membrane protein; Extracellular side. Membrane raft; Peripheral membrane protein; Extracellular side. Note=Localizes predominantly to azurophil granules (primary secretory granules) in neutrophils (PubMed:18462208, PubMed:2033050, PubMed:3198760, PubMed:7897245) Secreted upon neutrophil stimulation by TNF-alpha, lipopolysaccharide (LPS), fMLP and CXCL8/IL8 or during neutrophil transmigration (PubMed:22266279, PubMed:28240246). Following secretion tethered to the cell membrane by CD177 (PubMed:18462208, PubMed:22266279) |
| Tissue Location | Expressed in polymorphonuclear leukocytes (at protein level) (PubMed:2033050, PubMed:3198760, PubMed:7897245) Expressed in neutrophils (at protein level) (PubMed:17244676, PubMed:18462208, PubMed:21193407, PubMed:22266279, PubMed:28240246) Expressed in differentiating neutrophils (PubMed:18462208) |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
REFERENCES
1. Blood. 2007 Dec 1;110(12):4086-95. 2. Clin Exp Immunol. 2006 Jul;145(1):63-70.
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