HSP70 Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using HSP70 mouse mAb against Hela (1) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast cancer using HSP70 mouse mAb with DAB staining.
Figure 3: Immunofluorescence analysis of NIH/3T3 cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P34932
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5A6
Isotype	IgG1
Calculated MW	94331 Da
Description	This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Immunogen	Purified recombinant fragment of human HSP70 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Other Names	Heat shock 70 kDa protein 4, HSP70RY, Heat shock 70-related protein APG-2, HSPA4, APG2
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 ICC~~N/A E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	HSP70 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.