ERN1 Antibody
Purified Mouse Monoclonal Antibody
- 产品详情
- 实验流程
Application ![]()
| WB, IHC, E |
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Primary Accession | O75460 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 9F2 |
Isotype | IgG1 |
Calculated MW | 109735 Da |
Description | The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. |
Immunogen | Purified recombinant fragment of human ERN1(aa282-433) expressed in E. Coli. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
Gene ID | 2081 |
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Other Names | Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, 2.7.11.1, Endoribonuclease, 3.1.26.-, ERN1 (HGNC:3449) |
Dilution | WB~~1/500 - 1/2000 IHC~~1/500 - 1/2000 E~~N/A |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ERN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ERN1 (HGNC:3449) |
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Function | Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11175748, PubMed:11779464, PubMed:12637535, PubMed:19328063, PubMed:21317875, PubMed:28128204, PubMed:30118681, PubMed:36739529, PubMed:9637683). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:21317875, PubMed:24508390). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:21317875, PubMed:24508390). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed:21884936, PubMed:28067262). |
Cellular Location | Endoplasmic reticulum membrane; Single-pass type I membrane protein |
Tissue Location | Ubiquitously expressed. High levels observed in pancreatic tissue. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
REFERENCES
1. Biochem Biophys Res Commun. 2004 Apr 30;317(2):390-6. 2. Mol Cell Biol. 2005 Sep;25(17):7522-33. 3. Science. 2007 Nov 9;318(5852):944-9.

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