ERN1 Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using ERN1 mouse mAb against Raji (1), A431 (2), Jurkat (3), Hela(4) and HEK293 (5) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ERN1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded human Placenta
tissues using ERN1 mouse mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	O75460
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	9F2
Isotype	IgG1
Calculated MW	109735 Da
Description	The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.
Immunogen	Purified recombinant fragment of human ERN1(aa282-433) expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Other Names	Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, 2.7.11.1, Endoribonuclease, 3.1.26.-, ERN1 (HGNC:3449)
Dilution	WB~~1/500 - 1/2000 IHC~~1/500 - 1/2000 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ERN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.