THAP11 Antibody

Purified Mouse Monoclonal Antibody

Figure 1: Western blot analysis using THAP11 mouse mAb against Hela (1) and NTERA-2 (2) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and ovary cancer tissues (right) using THAP11 mouse mAb with DAB staining.
Figure 3: Immunofluorescence analysis of NTERA-2 cells using THAP11 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 4: Flow cytometric analysis of Hela cells using THAP11 mouse mAb (green) and negative control (purple).

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	Q96EK4
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3F3
Isotype	IgG1
Calculated MW	34455 Da
Description	The protein encoded by this gene contains a THAP domain, which is a conserved DNA-binding domain that has striking similarity to the site-specific DNA-binding domain (DBD) of Drosophila P element transposases.
Immunogen	Purified recombinant fragment of human THAP11 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	57215
Other Names	THAP domain-containing protein 11, THAP11
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	THAP11 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	THAP11
Function	Transcription factor, which has both transcriptional activation and repression activities (PubMed:31905202). Also modulates chromatin accessibility (PubMed:38361031). In complex with HCFC1 and ZNF143, regulates the expression of several genes, including AP2S1, ESCO2, OPHN1, RBL1, UBXN8 and ZNF32 (PubMed:26416877). May regulate the expression of genes that encode both cytoplasmic and mitochondrial ribosomal proteins (By similarity). Required for normal mitochondrial development and function. Regulates mitochondrial gene expression, including that of components of the electron transport chain (By similarity). Involved in the maintainance of pluripotency in early embryonic cells, possibly through its action on mitochondrial maturation which is required to meet high energy demands of these cells (By similarity). Required for early development of retina, preventing premature exit of retinal progenitor cells from the cell cycle. This effect may also be mediated by its action on mitochondria (By similarity). Through the regulation of MMACHC gene expression, controls cobalamin metabolism (PubMed:28449119, PubMed:31905202). Required for normal brain development and neural precursor differentiation (By similarity). Involved in cell growth (PubMed:31905202).
Cellular Location	Nucleus. Cytoplasm Note=In oocytes, detected in the ooplasm, without evidence of its presence in the nucleus (By similarity). Found in the nucleus of undifferentiated embryonic stem cells (PubMed:18585351). Evenly distributed between nucleus and cytoplasm in skin fibroblasts (PubMed:37148549). {ECO:0000250\|UniProtKB:Q9JJD0, ECO:0000269\|PubMed:18585351, ECO:0000269\|PubMed:37148549}
Tissue Location	Expressed in skin fibroblasts.