GRK2 Antibody

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining.
Figure 3: Immunofluorescence analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P25098
Reactivity	Human, Mouse, Rat, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	3F8
Isotype	IgG1
Calculated MW	79574 Da
Description	The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. (provided by RefSeq)Tissue specificity: Expressed in peripheral blood leukocytes
Immunogen	Purified recombinant fragment of human GRK2 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	156
Other Names	Beta-adrenergic receptor kinase 1, Beta-ARK-1, 2.7.11.15, G-protein coupled receptor kinase 2, ADRBK1, BARK, BARK1, GRK2
Dilution	WB~~1/500 - 1/2000 IHC~~1/500 - 1/2000 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GRK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GRK2 {ECO:0000303\|PubMed:40384633, ECO:0000312\|HGNC:HGNC:289}
Function	Specifically phosphorylates the activated forms of G protein- coupled receptors (PubMed:14976224, PubMed:16179383, PubMed:19306925, PubMed:19715378, PubMed:21799898, PubMed:23077507, PubMed:30284927, PubMed:39138140, PubMed:40384633). Such receptor phosphorylation initiates beta-arrestin-mediated receptor internalization, and signaling events leading to their desensitization (PubMed:14976224, PubMed:16179383, PubMed:19306925, PubMed:19715378, PubMed:21799898, PubMed:23077507, PubMed:30284927, PubMed:39138140, PubMed:40384633). Phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them (PubMed:19715378). Phosphorylates catecholamine-activated ADRB2 to regulate physiological cardiomyocyte contraction rate responses (By similarity). Also phosphorylates ligand-bound C3a and C5a anaphylatoxin receptors (C3AR1 and C5AR1, respectively), leading to receptor desensitization (PubMed:21799898, PubMed:23077507). Positively regulates ciliary smoothened (SMO) signaling pathway by mediating phosphorylation of SMO, facilitating SMO trafficking into the cilium and the stimulation of SMO activity (PubMed:39138140). Also positively regulates SMO signaling by mediating phosphorylation of GPR161, a key inhibitor SMO signaling, promoting ciliary export of GPR161 (PubMed:40384633). Key regulator of LPAR1 signaling (PubMed:19306925). Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor (PubMed:19306925). Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner (PubMed:19306925). Inhibits relaxation of airway smooth muscle in response to blue light (PubMed:30284927).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:P26817}. Cell membrane {ECO:0000250\|UniProtKB:P21146}. Cell projection, cilium membrane. Postsynapse {ECO:0000250\|UniProtKB:P26817}. Presynapse {ECO:0000250\|UniProtKB:P26817}
Tissue Location	Expressed in peripheral blood leukocytes.