ABCG2 Antibody

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using ABCG2 mouse mAb against HepG2 (1), Cos7 (2), Jurkat (3) and NIH/3T3 (4) cell lysate.
Figure 2: Immunofluorescence analysis of Hela cells using ABCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ICC, E
Primary Accession	Q9UNQ0
Reactivity	Human, Mouse, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	1H2
Isotype	IgG1
Calculated MW	72314 Da
Description	The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.
Immunogen	Purified recombinant fragment of human ABCG2 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Other Names	ATP-binding cassette sub-family G member 2, Breast cancer resistance protein, CDw338, Mitoxantrone resistance-associated protein, Placenta-specific ATP-binding cassette transporter, Urate exporter, CD338, ABCG2, ABCP, BCRP, BCRP1, MXR
Dilution	WB~~1/500 - 1/2000 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ABCG2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.