GKAP Antibody

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using GKAP mAb against HEK293 (1) and GKAP(AA: 490-663)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using GKAP mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded human esophagus tissues using GKAP mouse mAb with DAB staining.
Figure 4: Immunofluorescence analysis of NIH/3T3 cells using GKAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	O14490
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3G4
Isotype	IgG1
Calculated MW	108873 Da
Description	Function: Part of the postsynaptic scaffold in neuronal cells.Tissue specificity: Expressed in brain.
Immunogen	Purified recombinant fragment of human GKAP expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	9229
Other Names	Disks large-associated protein 1, DAP-1, Guanylate kinase-associated protein, hGKAP, PSD-95/SAP90-binding protein 1, SAP90/PSD-95-associated protein 1, SAPAP1, DLGAP1, DAP1, GKAP
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GKAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DLGAP1
Synonyms	DAP1, GKAP
Function	Part of the postsynaptic scaffold in neuronal cells.
Cellular Location	Cell membrane; Peripheral membrane protein. Postsynaptic density. Synapse
Tissue Location	Expressed in brain.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.