KIT Antibody

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using KIT mAb against human KIT (AA: 805-976) recombinant protein. (Expected MW is 45 kDa)
Figure 2: Western blot analysis using KIT mouse mAb against Jurkat (1) and Hela (2) cell lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded gastrointestinal stromal tumor using KIT mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P10721
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1C5
Isotype	IgG1
Calculated MW	109865 Da
Description	The c-Kit proto-oncogene is a member of the receptor tyrosine kinase family and, more specifically, is closely related to the platelet derived growth factor receptor (PDGFR). c-Kit, the normal cellular homolog of the HZ4-feline sarcoma virus transforming gene (v-Kit), encodes a transmembrane receptor. c-Kit regulates a variety of biological responses including chemotaxis, cell prolif- eration, apoptosis and adhesion. c-Kit is also identical with the product of the W locus in mice and, as such, is integral to the development of mast cells and hematopoiesis. The ligand for the c-Kit receptor (KL) has been identified and is encoded at the murine steel (SI) locus. Kit is the human homolog of the proto- oncogene c-Kit. Mutations in Kit are integral for tumor growth and progression in various cancers.
Immunogen	Purified recombinant fragment of human KIT expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Other Names	Mast/stem cell growth factor receptor Kit, SCFR, 2.7.10.1, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117, KIT, SCFR
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	KIT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.