HFE Antibody

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using HFE mAb against human HFE (AA: 125-282) recombinant protein. (Expected MW is 44 kDa)
Figure 2: Western blot analysis using HFE mAb against HEK293 (1) and HFE(AA: 125-282)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Immunofluorescence analysis of HepG2 cells using HFE mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ICC, E
Primary Accession	Q30201
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3F1
Isotype	IgG1
Calculated MW	40108 Da
Description	The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.
Immunogen	Purified recombinant fragment of human HFE expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	3077
Other Names	Hereditary hemochromatosis protein, HLA-H, HFE, HLAH
Dilution	WB~~1/500 - 1/2000 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	HFE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	HFE
Synonyms	HLAH
Function	Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Expressed in all tissues tested except brain.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.