MAP3K2 Antibody

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using MAP3K2 mAb against human MAP3K2 (AA: 148-359) recombinant protein. (Expected MW is 49.2 kDa)
Figure 2: Western blot analysis using MAP3K2 mAb against HEK293 (1) and MAP3K2(AA: 148-359)--hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Immunofluorescence analysis of 3T3-L1 cells using MAP3K2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ICC, E
Primary Accession	Q9Y2U5
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	4B4
Isotype	IgG1
Calculated MW	69741 Da
Description	The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process.
Immunogen	Purified recombinant fragment of human MAP3K2 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Gene ID	10746
Other Names	Mitogen-activated protein kinase kinase kinase 2, 2.7.11.25, MAPK/ERK kinase kinase 2, MEK kinase 2, MEKK 2, MAP3K2, MAPKKK2, MEKK2
Dilution	WB~~1/500 - 1/2000 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	MAP3K2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	MAP3K2
Synonyms	MAPKKK2, MEKK2
Function	Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and- run dynamics.
Cellular Location	Cytoplasm. Nucleus. Note=Upon EGF stimulation, translocates into the nucleus

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.