SRC Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using SRC mAb against human SRC (AA: 1-189) recombinant protein. (Expected MW is 47.8 kDa)
Figure 2: Western blot analysis using SRC mouse mAb against MCF-7 (1), A431 (2), Hela (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and Cos7 (7) cell lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SRC mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal tissues using SRC mouse mAb with DAB staining.
Figure 5: Flow cytometric analysis of MCF-7 cells using SRC mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	P12931
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1F11
Isotype	IgG1
Calculated MW	59835 Da
Description	This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Immunogen	Purified recombinant fragment of human SRC expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Additional Information
Other Names	Proto-oncogene tyrosine-protein kinase Src, 2.7.10.2, Proto-oncogene c-Src, pp60c-src, p60-Src, SRC, SRC1
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SRC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.