CYP1A1 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using CYP1A1 mAb against human CYP1A1 (AA: 203-461) recombinant protein. (Expected MW is 60 kDa)
Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CYP1A1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CYP1A1 mouse mAb with DAB staining.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P04798
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	6G5
Isotype	IgG1
Calculated MW	58165 Da
Description	This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15.
Immunogen	Purified recombinant fragment of human CYP1A1 expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	1543
Other Names	Cytochrome P450 1A1, 1.14.14.1, CYPIA1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1, CYP1A1
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CYP1A1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CYP1A1 {ECO:0000303\|PubMed:10681376, ECO:0000312\|HGNC:HGNC:2595}
Function	A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15041462, PubMed:15805301, PubMed:18577768, PubMed:19965576, PubMed:20972997). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15041462, PubMed:15805301, PubMed:18577768, PubMed:19965576, PubMed:20972997). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16- alpha positions (PubMed:11555828, PubMed:12865317, PubMed:14559847, PubMed:15805301). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed:15041462, PubMed:18577768). Catalyzes the epoxidation of double bonds of certain PUFA (PubMed:15041462, PubMed:19965576, PubMed:20972997). Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed:20972997). Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed:15041462). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195).
Cellular Location	Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:P00185}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P00185}. Mitochondrion inner membrane {ECO:0000250\|UniProtKB:P00185}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P00185}. Microsome membrane {ECO:0000250\|UniProtKB:P00185}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P00185}. Cytoplasm {ECO:0000250\|UniProtKB:P00185}
Tissue Location	Lung, lymphocytes and placenta.