ASS1 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using ASS1 mAb against human ASS1 (AA: 40-236) recombinant protein. (Expected MW is 47 kDa)
Figure 2: Western blot analysis using ASS1 mouse mAb against A431 (1), RAJI (2), MOLT4 (3), Jurkat (4), A549 (5), NIH/3T3 (6), PC-12 (7) and Cos7 (8) cell lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ASS1 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ASS1 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P00966
Reactivity	Human, Mouse, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	2C10
Isotype	IgG1
Calculated MW	46530 Da
Description	The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
Immunogen	Purified recombinant fragment of human ASS1 expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	445
Other Names	Argininosuccinate synthase, 6.3.4.5, Citrulline--aspartate ligase, ASS1, ASS
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ASS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ASS1 (HGNC:758)
Function	One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Cellular Location	Cytoplasm, cytosol
Tissue Location	Expressed in adult liver.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.