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CCL2 Antibody

Purified Mouse Monoclonal Antibody

     
  • 10 - CCL2 Antibody AO1701a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • 1 - CCL2 Antibody AO1701a
    Figure 1: Western blot analysis using CCL2 mAb against human CCL2 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa)
  • 1 - CCL2 Antibody AO1701a
    Figure 2: Western blot analysis using CCL2 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6)cell lysate.
  • 2 - CCL2 Antibody AO1701a
    Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CCL2 mouse mAb with DAB staining.
  • 3 - CCL2 Antibody AO1701a
    Figure 4: Immunofluorescence analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • 3 - CCL2 Antibody AO1701a
    Figure 5: Immunofluorescence analysis of HepG2 cells using CCL2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • 4 - CCL2 Antibody AO1701a
    Figure 6: Flow cytometric analysis of A549 cells using CCL2 mouse mAb (green) and negative control (red).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, FC, ICC, E
Primary Accession P13500
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 2D8
Isotype IgG1
Calculated MW 11025 Da
Description This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
Immunogen Purified recombinant fragment of human CCL2 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 6347
Other Names C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte chemotactic protein 1, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2, CCL2, MCP1, SCYA2
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
ICC~~N/A
E~~1/10000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCL2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCL2
Synonyms MCP1, SCYA2
Function Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:10529171, PubMed:10587439, PubMed:9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:10587439, PubMed:9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8195247, PubMed:8627182, PubMed:9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690).
Cellular Location Secreted
Tissue Location Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level) (PubMed:23765988). Expressed in monocytes (PubMed:2513477).
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

J Cereb Blood Flow Metab. 2010 Mar;30(3):459-73. Prostate. 2010 Mar 1;70(4):433-42.

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