SRY Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using SRY mAb against human SRY recombinant protein. (Expected MW is 49.4 kDa)
Figure 2: Western blot analysis using SRY mouse mAb against NTERA-2 (1) cell lysate.
Figure 3: Flow cytometric analysis of HepG2 cells using SRY mouse mAb (green) and negative control (red).
Figure 4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SRY mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SRY mouse mAb with DAB staining.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	Q05066
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1G4
Isotype	IgG1
Calculated MW	23884 Da
Description	This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome.
Immunogen	Purified recombinant fragment of human SRY (AA:1-204) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	6736
Other Names	Sex-determining region Y protein, Testis-determining factor, SRY, TDF
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SRY Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SRY {ECO:0000303\|PubMed:1695712, ECO:0000312\|HGNC:HGNC:11311}
Function	Transcriptional regulator that controls a genetic switch in male development (PubMed:11563911). It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (PubMed:16414182, PubMed:16996051). Involved in different aspects of gene regulation including promoter activation or repression (PubMed:9525897). Binds to the DNA consensus sequence 5'- [AT]AACAA[AT]-3' (PubMed:11563911, PubMed:1425584, PubMed:15170344, PubMed:8159753, PubMed:8265659). SRY HMG box recognizes DNA by partial intercalation in the minor groove and promotes DNA bending (PubMed:11563911, PubMed:1425584, PubMed:15170344, PubMed:16762365, PubMed:8159753, PubMed:8265659). Also involved in pre-mRNA splicing (PubMed:11818535). In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity).
Cellular Location	Nucleus speckle. Cytoplasm Nucleus. Note=Acetylation contributes to its nuclear localization and deacetylation by HDAC3 induces a cytoplasmic delocalization (PubMed:15297880). Colocalizes with SOX6 in speckles (PubMed:11818535). Colocalizes with CAML in the nucleus (PubMed:15746192). Colocalizes in the nucleus with ZNF208 isoform KRAB- O and tyrosine hydroxylase (TH) (By similarity). Nuclear import is facilitated by XPO4, a protein that usually acts as a nuclear export signal receptor (PubMed:19349578). {ECO:0000250\|UniProtKB:Q05738, ECO:0000269\|PubMed:11818535, ECO:0000269\|PubMed:15297880, ECO:0000269\|PubMed:15746192, ECO:0000269\|PubMed:19349578}