UTF1 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using UTF1 mAb against human UTF1 recombinant protein. (Expected MW is 32.8 kDa)
Figure 2: Western blot analysis using UTF1 mAb against HEK293 (1) and UTF1 (AA: 148-214)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Flow cytometric analysis of K562 cells using UTF1 mouse mAb (green) and negative control (purple).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q5T230
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5B6
Isotype	IgG1
Calculated MW	36439 Da
Description	Undifferentiated transcription factor-1 (UTF-1) is used as marker for the undifferentiated state of pluripotent stem cells.UTF1 is a chromatin-associated protein with core histone-like properties. UTF1 further acts as a transcriptional repressor and is required for proper differentiation of pluripotent cells.
Immunogen	Purified recombinant fragment of human UTF1 (AA: 148-214) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	8433
Other Names	Undifferentiated embryonic cell transcription factor 1, UTF1 (HGNC:12634)
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	UTF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.