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Ring1 Antibody

Purified Mouse Monoclonal Antibody

     
  • 10 - Ring1 Antibody AO1804a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • 1 - Ring1 Antibody AO1804a
    Figure 1: Western blot analysis using Ring1 mAb against human Ring1 recombinant protein. (Expected MW is 44.6 kDa)
  • 1 - Ring1 Antibody AO1804a
    Figure 2: Western blot analysis using Ring1 mAb against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.
  • 1 - Ring1 Antibody AO1804a
    Figure 3: Western blot analysis using Ring1 mouse mAb against MOLT-4 (1), LNCaP (2), Hela (3), HEK-293 (4) and Jurkat (5) cell lysate.
  • 4 - Ring1 Antibody AO1804a
    Figure 4: Flow cytometric analysis of Hela cells using Ring1 mouse mAb (green) and negative control (red).
  • 2 - Ring1 Antibody AO1804a
    Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Ring1 mouse mAb with DAB staining.
  • 2 - Ring1 Antibody AO1804a
    Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using Ring1 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, FC, E
Primary Accession Q06587
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8C12F4
Isotype IgG1
Calculated MW 42429 Da
Description This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4.
Immunogen Purified recombinant fragment of human Ring1 (AA: 79-263) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 6015
Other Names E3 ubiquitin-protein ligase RING1, 6.3.2.-, Polycomb complex protein RING1, RING finger protein 1, Really interesting new gene 1 protein, RING1, RNF1
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
E~~1/10000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRing1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RING1 (HGNC:10018)
Function Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
Cellular Location Nucleus. Nucleus speckle
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.

REFERENCES

1.Int J Dev Biol. 2009;53(2-3):355-70. 2.PLoS One. 2009 Dec 1;4(12):e8104.

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