CD7 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using CD7 mAb against human CD7 recombinant protein. (Expected MW is 36.6 kDa)
Figure 2: Western blot analysis using CD7 mAb against HEK293 (1) and CD7 (AA: 72-175)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Flow cytometric analysis of Jurkat cells using CD7 mouse mAb (green) and negative control (red).
Figure 5: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CD7 mouse mAb with DAB staining.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	P09564
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	4D4F8
Isotype	IgG1
Calculated MW	25409 Da
Description	This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
Immunogen	Purified recombinant fragment of human CD7 (AA: 72-175) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	924
Other Names	T-cell antigen CD7, GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, TP41, CD7, CD7
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CD7
Function	Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors (PubMed:7506726). Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1 (PubMed:10652336). In turn, mediates the production of cytokines such as IL-2 (PubMed:1709867). On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels (PubMed:7506726).
Cellular Location	Membrane; Single-pass type I membrane protein.
Tissue Location	Expressed on T-cells and natural killer (NK) cells and their precursors.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

REFERENCES

1.Mol Cancer. 2010 Feb 22;9:41. 2.J Hematol Oncol. 2010 Apr 14;3:15.

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¥ 2,600.00

Cat# AO1806a

Size:

100 µl

Price:

¥ 2,600.00

Quantity:

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Availability: 3-5 days

询价

CD7 Antibody

Purified Mouse Monoclonal Antibody

BACKGROUND

REFERENCES

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