ACP5 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using ACP5 mAb against human ACP5 recombinant protein. (Expected MW is 37.3 kDa)
Figure 2: Western blot analysis using ACP5 mAb against HEK293 (1) and ACP5 (AA: 221-325)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using ACP5 mouse mAb against A431 (1), T47D (2), HepG2 (3), MOLT4 (4), Jurkat (5) and Hela (6) cell lysate.
Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ACP5 mouse mAb with DAB staining.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P13686
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7E6A11
Isotype	IgG1
Calculated MW	36599 Da
Description	This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.
Immunogen	Purified recombinant fragment of human ACP5 (AA: 221-325) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	54
Other Names	Tartrate-resistant acid phosphatase type 5, TR-AP, 3.1.3.2, Tartrate-resistant acid ATPase, TrATPase, Type 5 acid phosphatase, ACP5
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ACP5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ACP5
Function	Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T- cell leukemias.
Cellular Location	Lysosome.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. ;

REFERENCES

1. Clin Chim Acta. 2011 May 12;412(11-12):963-9. 2. Eur J Gynaecol Oncol. 2011;32(6):615-8.

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¥ 2,600.00

Cat# AO1841a

Size:

100 µl

Price:

¥ 2,600.00

Quantity:

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Availability: 3-5 days

询价

ACP5 Antibody

Purified Mouse Monoclonal Antibody

BACKGROUND

REFERENCES

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