MEN1 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using MEN1 mAb against human MEN1 (AA: 392-554) recombinant protein. (Expected MW is 43.3 kDa)
Figure 2: Western blot analysis using MEN1 mAb against HEK293 (1) and MEN1 (AA: 392-554)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Flow cytometric analysis of Hela cells using MEN1 mouse mAb (green) and negative control (red).
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MEN1 mouse mAb with DAB staining.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	O00255
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7D3E10
Isotype	IgG1
Calculated MW	68023 Da
Description	This gene encodes menin, a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of this protein is not known. Two messages have been detected on northern blots but the larger message has not been characterized. Alternative splicing results in multiple transcript variants.
Immunogen	Purified recombinant fragment of human MEN1 (AA: 392-554) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Additional Information
Other Names	Menin, MEN1, SCG2
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	MEN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

The innate immune system confers host defense against viral and microbial infection, and TRAFD1 is a negative feedback regulator that controls excessive immune responses ; ;

REFERENCES

1. Clinics (Sao Paulo). 2012;67 Suppl 1:49-56.2. World J Surg Oncol. 2011 Jan 25;9:6.

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¥ 1,250.00

Cat# AO1941a

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

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Availability: 3-5 days

询价

MEN1 Antibody

Purified Mouse Monoclonal Antibody

BACKGROUND

REFERENCES

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