VTN Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using VTN mAb against human VTN (AA: 20-199) recombinant protein. (Expected MW is 45.9 kDa)
Figure 2: Western blot analysis using VTN mAb against HEK293 (1) and VTN (AA: 20-199)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Flow cytometric analysis of Hela cells using VTN mouse mAb (green) and negative control (red).
Figure 4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using VTN mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using VTN mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	P04004
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1G11E8
Isotype	IgG2b
Calculated MW	54306 Da
Description	The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
Immunogen	Purified recombinant fragment of human VTN (AA: 20-199) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Additional Information
Gene ID	7448
Other Names	Vitronectin, VN, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B, VTN
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	VTN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	VTN
Function	Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Cellular Location	Secreted, extracellular space
Tissue Location	Expressed in the retina pigment epithelium (at protein level) (PubMed:25136834). Expressed in plasma (at protein level) (PubMed:2448300). Expressed in serum (at protein level) (PubMed:29567995).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.