PER3 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using PER3 mAb against human PER3 (AA: 723-954) recombinant protein. (Expected MW is 50.7 kDa)
Figure 2: Western blot analysis using PER3 mAb against HEK293 (1) and PER3 (AA: 723-954)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Immunofluorescence analysis of Hela cells using PER3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4: Flow cytometric analysis of Hela cells using PER3 mouse mAb (green) and negative control (red).
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PER3 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PER3 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	P56645
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	4B9D7
Isotype	IgG2b
Calculated MW	131888 Da
Description	This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known.
Immunogen	Purified recombinant fragment of human PER3 (AA: 723-954) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Additional Information
Other Names	Period circadian protein homolog 3, hPER3, Cell growth-inhibiting gene 13 protein, Circadian clock protein PERIOD 3, PER3
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PER3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.