GSK3B Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using GSK3B mAb against human GSK3B (AA: 2-159) recombinant protein. (Expected MW is 20.3 kDa)
Figure 2: Flow cytometric analysis of NIH/3T3 cells using GSK3B mouse mAb (green) and negative control (red).
Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using GSK3B mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GSK3B mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	P49841
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Clone Names	3D6B4
Isotype	IgG1
Calculated MW	46744 Da
Description	The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen	Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Additional Information
Other Names	Glycogen synthase kinase-3 beta, GSK-3 beta, 2.7.11.26, Serine/threonine-protein kinase GSK3B, 2.7.11.1, GSK3B
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GSK3B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.