MuRF1 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1:Immunofluorescence analysis of Hela cells using MuRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 2: Flow cytometric analysis of Hela cells using MuRF1 mouse mAb (green) and negative control (red).
Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MuRF1 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded muscle tissues using MuRF1 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC, FC, ICC, E
Primary Accession	Q969Q1
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7B8E1
Isotype	IgG1
Calculated MW	40248 Da
Description	This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase.
Immunogen	Synthesized peptide of human MuRF1 (AA: 293-304).
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Additional Information
Gene ID	84676
Other Names	E3 ubiquitin-protein ligase TRIM63, 6.3.2.-, Iris RING finger protein, Muscle-specific RING finger protein 1, MuRF-1, MuRF1, RING finger protein 28, Striated muscle RING zinc finger protein, Tripartite motif-containing protein 63, TRIM63, IRF, MURF1, RNF28, SMRZ
Dilution	IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	MuRF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TRIM63
Synonyms	IRF, MURF1, RNF28, SMRZ
Function	E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Cellular Location	Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line Note=Colocalizes with TNNI3 in myocytes (By similarity). Localizes to the M- and Z-lines in skeletal muscle.
Tissue Location	Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.