CD6 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using CD6 mAb against human CD6 (AA: Extra(18-199)) recombinant protein. (Expected MW is 44.8 kDa)
Figure 2: Western blot analysis using CD6 mAb against HEK293 (1) and CD6 (AA: Extra(18-199))-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Flow cytometric analysis of Hela cells using CD6 mouse mAb (green) and negative control (red).
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD6 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD6 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	P30203
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	6B11G6
Isotype	IgG2b
Calculated MW	71801 Da
Description	This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis (PMID: 19525953, 21849685). Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen	Purified recombinant fragment of human CD6 (AA: Extra(18-199)) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Additional Information
Other Names	T-cell differentiation antigen CD6, T12, TP120, CD6, CD6
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.