TFF2 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using TFF2 mAb against human TFF2 (AA: 20-125) recombinant protein. (Expected MW is 37.3 kDa)
Figure 2: Western blot analysis using TFF2 mAb against HEK293 (1) and TFF2 (AA: 20-125)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Flow cytometric analysis of Hela cells using TFF2 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q03403
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	4G7C3
Isotype	IgG1
Calculated MW	14284 Da
Description	Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
Immunogen	Purified recombinant fragment of human TFF2 (AA: 20-125) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Additional Information
Gene ID	7032
Other Names	Trefoil factor 2, Spasmolysin, Spasmolytic polypeptide, SP, TFF2, SML1
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TFF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TFF2
Synonyms	SML1
Function	Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains (By similarity).
Cellular Location	Secreted.
Tissue Location	Stomach.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.