CD38 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using CD38 mAb against human CD38 (AA: Extra(43-300)) recombinant protein. (Expected MW is 56.1 kDa)
Figure 2: Western blot analysis using CD38 mAb against HEK293 (1) and CD38 (AA: Extra(43-300))-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using CD38 mouse mAb against HEK293 (1), LNcap (2) cell lysate.
Figure 4: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using CD38 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P28907
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5C5C3
Isotype	IgG1
Calculated MW	34328 Da
Description	CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling.
Immunogen	Purified recombinant fragment of human CD38 (AA: Extra(43-300)) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Additional Information
Gene ID	952
Other Names	ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 3.2.2.6, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2.4.99.20, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, ADPRC 1, Cyclic ADP-ribose hydrolase 1, cADPr hydrolase 1, T10, CD38, CD38
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD38 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CD38
Function	Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed:7961800, PubMed:8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed:16690024). Has cADPR hydrolase activity (PubMed:7961800, PubMed:8253715).
Cellular Location	Cell surface. Membrane; Single-pass type II membrane protein
Tissue Location	Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.