VAV2 Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using VAV2 mAb against human VAV2 (AA: 552-868) recombinant protein. (Expected MW is *** kDa)
Figure 3:Western blot analysis using VAV2 mAb against HEK293 (1) and VAV2 (AA: 552-868)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Hela cells using VAV2 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	P52735
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3F5D3
Isotype	IgG1
Calculated MW	101289 Da
Description	VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen	Purified recombinant fragment of human VAV2 (AA: 552-868) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	7410
Other Names	Guanine nucleotide exchange factor VAV2, VAV-2, VAV2
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	VAV2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	VAV2
Function	Guanine nucleotide exchange factor for the Rho family of Ras- related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity).
Tissue Location	Widely expressed.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.