ADD1 Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using ADD1 mAb
against human ADD1 (AA: 1-193)
recombinant protein. (Expected MW is 47.8 kDa)
Figure 3:Western blot analysis using ADD1 mAb
against HEK293 (1) and ADD1 (AA: 1-193)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using 1-193 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using 1-193 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P35611
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5D4H1
Isotype	IgG1
Calculated MW	80955 Da
Description	Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Immunogen	Purified recombinant fragment of human ADD1 (AA: 1-193) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	118
Other Names	Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ADD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ADD1
Synonyms	ADDA
Function	Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Cellular Location	Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location	Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.