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HSPB2 Antibody

Purified Mouse Monoclonal Antibody

     
  • 1 - HSPB2 Antibody AO2045a

    Figure 1:Western blot analysis using HSPB2 mAb against human HSPB2 (AA: 1-182) recombinant protein. (Expected MW is 46.2 kDa)

  • 10 - HSPB2 Antibody AO2045a

    Figure 2:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • 1 - HSPB2 Antibody AO2045a

    Figure 3:Western blot analysis using HSPB2 mAb against HEK293 (1) and HSPB2 (AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.

  • 4 - HSPB2 Antibody AO2045a

    Figure 4:Flow cytometric analysis of A549 cells using HSPB2 mouse mAb (green) and negative control (red).

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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q16082
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4B9E2
Isotype IgG1
Calculated MW 20233 Da
Description The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The protein is expressed preferentially in the heart and skeletal muscle. This protein regulates Myotonic Dystrophy Protein Kinase, which plays an important role in maintenance of muscle structure and function. [provided by RefSeq, Dec 2012]
Immunogen Purified recombinant fragment of human HSPB2 (AA: 1-182) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 3316
Other Names Heat shock protein beta-2, HspB2, DMPK-binding protein, MKBP, HSPB2
Dilution WB~~1/500 - 1/2000
FC~~1/200 - 1/400
E~~1/10000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPB2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPB2
Function May regulate the kinase DMPK.
Cellular Location Cytoplasm. Nucleus. Note=Localizes to nuclear foci
Tissue Location Expressed preferentially in skeletal muscle and heart but not in the lens
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

Int J Oncol. 2011 Apr;38(4):1129-35. J Heart Lung Transplant. 2010 Jul;29(7):786-91.

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