TTR Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using TTR mAb against human TTR (AA: 1-147) recombinant protein. (Expected MW is 45.8 kDa)
Figure 3:Western blot analysis using TTR mAb against HEK293 (1) and TTR (AA: 1-147)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of A549 cells using TTR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of MCF-7 cells using TTR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of A549 cells using TTR mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TTR mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	P02766
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2E10C5
Isotype	IgG1
Calculated MW	15887 Da
Description	This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc. [provided by RefSeq, Jan 2009]
Immunogen	Purified recombinant fragment of human TTR (AA: 1-147) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	7276
Other Names	Transthyretin, ATTR, Prealbumin, TBPA, TTR, PALB
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TTR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TTR
Synonyms	PALB
Function	Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Cellular Location	Secreted. Cytoplasm.
Tissue Location	Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells Detected in retina pigment epithelium and liver

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.