IRAK3 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using IRAK3 mAb against human IRAK3 (AA: 454-596) recombinant protein. (Expected MW is 42.3 kDa)
Figure 3:Western blot analysis using IRAK3 mAb against HEK293 (1) and IRAK3 (AA: 454-596)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of A549 cells using IRAK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using IRAK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of HepG2 cells using IRAK3 mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using IRAK3 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	Q9Y616
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	6G9G2
Isotype	IgG1
Calculated MW	67767 Da
Description	This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants.
Immunogen	Purified recombinant fragment of human IRAK3 (AA: 454-596) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	11213
Other Names	Interleukin-1 receptor-associated kinase 3, IRAK-3, 2.7.11.1, IL-1 receptor-associated kinase M, IRAK-M, IRAK3 {ECO:0000312\|EMBL:AAH57800.1}
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	IRAK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	IRAK3 {ECO:0000312\|EMBL:AAH57800.1}
Function	Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors (PubMed:10383454, PubMed:29686383). Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex (By similarity). Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells (PubMed:29686383).
Cellular Location	Cytoplasm. Nucleus. Note=In dendritic cells, translocates into the nucleus upon IL33 stimulation. {ECO:0000250\|UniProtKB:Q8K4B2}
Tissue Location	Expressed in eosinophils, dendritic cells and/or monocytes (at protein level) (PubMed:29686383). Expressed predominantly in peripheral blood lymphocytes (PubMed:10383454)

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.