IRAK3 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using IRAK3 mAb against human IRAK3 (AA: 454-596) recombinant protein. (Expected MW is 42.3 kDa)
Figure 3:Western blot analysis using IRAK3 mAb against HEK293 (1) and IRAK3 (AA: 454-596)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of A549 cells using IRAK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using IRAK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of HepG2 cells using IRAK3 mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using IRAK3 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	Q9Y616
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	6G9G2
Isotype	IgG1
Calculated MW	67767 Da
Description	This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants.
Immunogen	Purified recombinant fragment of human IRAK3 (AA: 454-596) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	11213
Other Names	Interleukin-1 receptor-associated kinase 3, IRAK-3, 2.7.11.1, IL-1 receptor-associated kinase M, IRAK-M, IRAK3 {ECO:0000312\|EMBL:AAH57800.1}
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	IRAK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	IRAK3 {ECO:0000312\|EMBL:AAH57800.1}
Function	Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors (PubMed:10383454, PubMed:29686383). Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex (By similarity). Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells (PubMed:29686383).
Cellular Location	Cytoplasm. Nucleus. Note=In dendritic cells, translocates into the nucleus upon IL33 stimulation. {ECO:0000250\|UniProtKB:Q8K4B2}
Tissue Location	Expressed in eosinophils, dendritic cells and/or monocytes (at protein level) (PubMed:29686383). Expressed predominantly in peripheral blood lymphocytes (PubMed:10383454)

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.