EMD Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using EMD mAb against human EMD (AA: 1-222) recombinant protein. (Expected MW is 51.1 kDa)
Figure 3:Western blot analysis using EMD mAb against HEK293 (1) and EMD (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of Hela cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Flow cytometric analysis of A549 cells using EMD mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of K562 cells using EMD mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using EMD mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	P50402
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	8F5A8
Isotype	IgG1
Calculated MW	28994 Da
Description	Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
Immunogen	Purified recombinant fragment of human EMD (AA: 1-222) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	Emerin, EMD, EDMD, STA
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	EMD Antibody is for research use only and not for use in diagnostic or therapeutic procedures.