AIM2 Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Western blot analysis using AIM2 mAb against human AIM2 (AA: 1-195) recombinant protein. (Expected MW is 48.2 kDa)
Figure 2:Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 3:Western blot analysis using AIM2 mAb against HEK293 (1) and AIM2 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of A549 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of MCF-7 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of Hela cells using AIM2 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, ICC, E
Primary Accession	O14862
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3C4G11
Isotype	IgG1
Calculated MW	38954 Da
Description	AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.
Immunogen	Purified recombinant fragment of human AIM2 (AA: 1-195) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	Interferon-inducible protein AIM2, Absent in melanoma 2, AIM2
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	AIM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.