RAB11FIP1 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using RAB11FIP1 mAb against human RAB11FIP1 (AA: 130-271) recombinant protein. (Expected MW is 41.7 kDa)
Figure 3:Western blot analysis using RAB11FIP1 mAb against HEK293 (1) and RAB11FIP1 (AA: 130-271)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using RAB11FIP1 mouse mAb against Raji (1), SW620 (2), A431 (3), SW480 (4), HepG2 (5), Hela (6), and NIH3T3 (7) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using RAB11FIP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RAB11FIP1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAB11FIP1 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	Q6WKZ4
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Clone Names	1A12B9D8
Isotype	IgG1
Calculated MW	137167 Da
Description	This gene encodes one of the Rab11-family interacting proteins (Rab11-FIPs), which play a role in the Rab-11 mediated recycling of vesicles. The encoded protein may be involved in endocytic sorting, trafficking of proteins including integrin subunits and epidermal growth factor receptor (EGFR), and transport between the recycling endosome and the trans-Golgi network. Alternative splicing results in multiple transcript variants. A pseudogene is described on the X chromosome
Immunogen	Purified recombinant fragment of human RAB11FIP1 (AA: 130-271) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	80223
Other Names	Rab11 family-interacting protein 1, Rab11-FIP1, Rab-coupling protein, RAB11FIP1, RCP
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1:10~50 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RAB11FIP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RAB11FIP1 (HGNC:30265)
Synonyms	RCP
Function	A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis. Interaction with RAB14 may function in the process of neurite formation (PubMed:26032412).
Cellular Location	Recycling endosome. Cytoplasmic vesicle. Note=RAB11A rather than RAB4A mediates localization in the endocytic recycling compartment (ERC) Colocalizes with Rab11 and RAB14 on punctate vesicles (PubMed:26032412).
Tissue Location	Isoform 2 is expressed in brain, heart, testis, lung, spleen, ovary and small intestine.