AOF1 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using AOF1 mAb against human AOF1 (AA: 6-129) recombinant protein. (Expected MW is 40 kDa)
Figure 3:Western blot analysis using AOF1 mAb against HEK293 (1) and AOF1 (AA: 6-129)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of HeLa cells using AOF1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 5:Immunofluorescence analysis of HeLa cells using AOF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using AOF1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using AOF1 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	Q8NB78
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5E10C1
Isotype	IgG1
Calculated MW	92098 Da
Description	Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function.
Immunogen	Purified recombinant fragment of human AOF1 (AA: 6-129) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	221656
Other Names	Lysine-specific histone demethylase 1B, 1.-.-.-, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 2, KDM1B, AOF1, C6orf193, LSD2
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1:10~50 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	AOF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	KDM1B (HGNC:21577)
Function	Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes (PubMed:30970244).
Cellular Location	Nucleus. Chromosome. Note=Found in actively RNAPolII- transcribed gene bodies.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.